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Best Buttermilk Substitute in Biscuits

5 tested options with exact ratios, science-backed explanations, and tips for success.

Quick Answer

The best substitute for Buttermilk in Biscuits is Milk + Lemon Juice or Vinegar because it replicates the acidity and moisture content of buttermilk, which is essential for activating baking soda and creating tender, flaky biscuits.

Top 5 Buttermilk Substitutes for Biscuits

Substitute Ratio
Milk + Lemon Juice or Vinegar (Best) 1 cup milk + 1 tablespoon lemon juice or white vinegar (let sit 5 minutes)
Plain Yogurt (thinned with water or milk) 3/4 cup plain yogurt + 1/4 cup water or milk per 1 cup buttermilk
Sour Cream + Water 3/4 cup sour cream + 1/4 cup water per 1 cup buttermilk
Milk + Cream of Tartar 1 cup milk + 1 1/2 teaspoons cream of tartar
Vegan Buttermilk (Plant Milk + Acid) 1 cup unsweetened soy or oat milk + 1 tablespoon lemon juice or apple cider vinegar

Detailed Guide: Each Buttermilk Substitute in Biscuits

Milk + Lemon Juice or Vinegar (Best Option)

1 cup milk + 1 tablespoon lemon juice or white vinegar (let sit 5 minutes)
Nut-Free
Quick tip: Mimics buttermilk acidity and moisture closely, maintaining biscuit rise and tenderness.

This substitute works because the acid (lemon juice or vinegar) curdles the milk slightly, lowering the pH to simulate buttermilk’s acidity. This acidity is crucial for activating baking soda, which produces carbon dioxide gas that leavens the biscuits.

For best results, use whole or 2% milk and allow the acid to react for at least 5 minutes before mixing into the dough. Avoid using skim milk as it reduces fat content, affecting tenderness.

Compared to original buttermilk, this substitute produces very similar texture and rise, with only a slight difference in flavor that is generally undetectable in biscuits.

Plain Yogurt (thinned with water or milk)

3/4 cup plain yogurt + 1/4 cup water or milk per 1 cup buttermilk
Gluten-Free Nut-Free
Quick tip: Provides acidity and moisture but requires thinning to avoid overly dense dough.

Plain yogurt contains lactic acid similar to buttermilk, which helps activate baking soda for leavening. However, yogurt is thicker and more concentrated, so thinning it with water or milk adjusts the consistency to better match buttermilk.

Mix well to ensure uniform texture and avoid lumps. Use full-fat yogurt for best tenderness and flavor.

This substitute yields biscuits with good rise and a slightly tangier flavor. The texture may be a bit denser if thinning is insufficient.

Sour Cream + Water

3/4 cup sour cream + 1/4 cup water per 1 cup buttermilk
Gluten-Free Nut-Free
Quick tip: Adds acidity and fat but must be diluted to prevent heaviness in biscuits.

Sour cream has acidity and fat content similar to buttermilk, which helps with leavening and tenderness. Diluting with water reduces thickness, making it easier to incorporate into biscuit dough.

Stir thoroughly to achieve a smooth consistency. Use full-fat sour cream for best results.

Biscuits made with this substitute tend to be rich and tender but may be slightly denser and heavier than those made with buttermilk.

Milk + Cream of Tartar

1 cup milk + 1 1/2 teaspoons cream of tartar
Gluten-Free Nut-Free
Quick tip: Provides acidity but can impart a slight aftertaste if overused.

Cream of tartar is an acid that can lower the pH of milk to mimic buttermilk’s acidity, activating baking soda for leavening. This combination replicates the chemical environment but lacks the flavor complexity of cultured buttermilk.

Mix cream of tartar thoroughly into milk before adding to dough. Avoid adding more than recommended to prevent off-flavors.

Biscuits will rise well and have a tender crumb, but the flavor may be less tangy and slightly metallic if cream of tartar is overused.

Vegan Buttermilk (Plant Milk + Acid)

1 cup unsweetened soy or oat milk + 1 tablespoon lemon juice or apple cider vinegar
Vegan Dairy-Free Nut-Free
Quick tip: Suitable for dairy-free diets, but may alter flavor and texture slightly.

Plant-based milks combined with acid create a vegan buttermilk substitute that provides acidity for leavening. Soy and oat milks have protein content closer to dairy milk, helping with structure.

Allow the mixture to sit for 5 minutes before use to curdle properly. Avoid sweetened or flavored plant milks.

Biscuits made with this substitute may be slightly less tender and have a different flavor profile, but still achieve good rise and flakiness.

Vegan Buttermilk Substitutes for Biscuits

Full Vegan guide →
Vegan Buttermilk (Plant Milk + Acid)
Ratio: 1 cup unsweetened soy or oat milk + 1 tablespoon lemon juice or apple cider vinegar

Suitable for dairy-free diets, but may alter flavor and texture slightly.

Gluten-Free Buttermilk Substitutes for Biscuits

Full Gluten-Free guide →
Plain Yogurt (thinned with water or milk)
Ratio: 3/4 cup plain yogurt + 1/4 cup water or milk per 1 cup buttermilk

Provides acidity and moisture but requires thinning to avoid overly dense dough.

Sour Cream + Water
Ratio: 3/4 cup sour cream + 1/4 cup water per 1 cup buttermilk

Adds acidity and fat but must be diluted to prevent heaviness in biscuits.

Milk + Cream of Tartar
Ratio: 1 cup milk + 1 1/2 teaspoons cream of tartar

Provides acidity but can impart a slight aftertaste if overused.

Dairy-Free Buttermilk Substitutes for Biscuits

Full Dairy-Free guide →
Vegan Buttermilk (Plant Milk + Acid)
Ratio: 1 cup unsweetened soy or oat milk + 1 tablespoon lemon juice or apple cider vinegar

Suitable for dairy-free diets, but may alter flavor and texture slightly.

❌ What NOT to Use as a Buttermilk Substitute in Biscuits

Plain Milk

Plain milk lacks the necessary acidity to react with baking soda, resulting in biscuits that are denser and less tender with reduced rise.

Yogurt (without thinning)

Using thick yogurt without thinning can lead to overly dense biscuits due to excess thickness and insufficient moisture, disrupting the dough consistency.

Sour Cream

Sour cream’s higher fat content and thickness can make biscuit dough too heavy and greasy, negatively affecting the light, flaky texture desired.

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