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Best Buttermilk Substitute in Cupcakes

5 tested options with exact ratios, science-backed explanations, and tips for success.

Quick Answer

The best substitute for Buttermilk in Cupcakes is Milk + Lemon Juice or Vinegar because it replicates the acidity and moisture content of buttermilk, which is essential for proper leavening and tender crumb formation.

Top 5 Buttermilk Substitutes for Cupcakes

Substitute Ratio
Milk + Lemon Juice or Vinegar (Best) 1 cup milk + 1 tablespoon lemon juice or white vinegar (let sit 5-10 minutes)
Plain Yogurt (thinned with water or milk) 3/4 cup plain yogurt + 1/4 cup water or milk per 1 cup buttermilk
Sour Cream (thinned with water or milk) 3/4 cup sour cream + 1/4 cup water or milk per 1 cup buttermilk
Evaporated Milk + Lemon Juice or Vinegar 1 cup evaporated milk + 1 tablespoon lemon juice or white vinegar (let sit 5-10 minutes)
Non-Dairy Milk (Almond, Soy) + Lemon Juice or Vinegar 1 cup non-dairy milk + 1 tablespoon lemon juice or white vinegar (let sit 5-10 minutes)

Detailed Guide: Each Buttermilk Substitute in Cupcakes

Milk + Lemon Juice or Vinegar (Best Option)

1 cup milk + 1 tablespoon lemon juice or white vinegar (let sit 5-10 minutes)
Nut-Free
Quick tip: Provides similar acidity and moisture to buttermilk, preserving cupcake rise and tenderness.

This substitute works because the acid (lemon juice or vinegar) curdles the milk slightly, mimicking the acidity and texture of buttermilk. The acid reacts with baking soda in the cupcake batter to produce carbon dioxide, which helps the cupcakes rise and creates a tender crumb.

For best results, use whole or 2% milk and allow the acid to sit in the milk for at least 5 minutes before adding to the batter. This ensures proper curdling and acidity. Avoid using skim milk as it may reduce richness.

Compared to original buttermilk, this substitute yields very similar texture and flavor, with only a slight difference in tanginess that is generally imperceptible in cupcakes.

Plain Yogurt (thinned with water or milk)

3/4 cup plain yogurt + 1/4 cup water or milk per 1 cup buttermilk
Gluten-Free Nut-Free
Quick tip: Thinning yogurt approximates buttermilk’s consistency and acidity, maintaining moisture and crumb softness.

Yogurt contains lactic acid similar to buttermilk, which reacts with baking soda to leaven cupcakes. However, yogurt is thicker, so thinning it with water or milk adjusts the viscosity to better match buttermilk, ensuring proper batter consistency and aeration.

Use plain, unsweetened yogurt and mix thoroughly with water or milk before incorporating. Over-thick batter can lead to dense cupcakes, so consistency is key.

This substitute produces cupcakes with a slightly tangier flavor and a moist, tender crumb, closely resembling those made with buttermilk.

Sour Cream (thinned with water or milk)

3/4 cup sour cream + 1/4 cup water or milk per 1 cup buttermilk
Gluten-Free Nut-Free
Quick tip: Thinning sour cream reduces density, helping maintain cupcake lightness while providing acidity.

Sour cream is acidic and rich, which can enhance flavor and tenderness. However, it is thicker and higher in fat than buttermilk, so thinning it with water or milk adjusts the texture to prevent overly dense cupcakes.

Mix sour cream with water or milk until pourable before adding to batter. Be cautious not to add too much liquid beyond the ratio to avoid thinning the batter excessively.

Cupcakes made with this substitute tend to be richer and moister, with a slightly tangy flavor, but may be denser than those made with buttermilk.

Evaporated Milk + Lemon Juice or Vinegar

1 cup evaporated milk + 1 tablespoon lemon juice or white vinegar (let sit 5-10 minutes)
Nut-Free
Quick tip: Provides acidity and richness but results in slightly denser cupcakes due to evaporated milk’s concentration.

Evaporated milk is concentrated milk with less water content, so when acidified, it mimics buttermilk’s acidity but adds more richness and density. The acid reacts with baking soda to leaven the cupcakes properly.

Allow the acid to sit in the evaporated milk for 5-10 minutes before use. Because evaporated milk is thicker, cupcakes may be slightly denser and richer than with buttermilk.

This substitute is useful when fresh milk is unavailable, but expect a minor difference in texture and flavor.

Non-Dairy Milk (Almond, Soy) + Lemon Juice or Vinegar

1 cup non-dairy milk + 1 tablespoon lemon juice or white vinegar (let sit 5-10 minutes)
Vegan Dairy-Free Nut-Free
Quick tip: Suitable dairy-free substitute that provides acidity but may impart slight nutty or bean flavor depending on milk used.

Non-dairy milks like almond or soy milk can be acidified with lemon juice or vinegar to replicate buttermilk’s acidity, which is necessary for activating baking soda in cupcakes. This maintains leavening and tenderness.

Let the acid sit in the non-dairy milk for at least 5 minutes before use. Be aware that some non-dairy milks have distinct flavors that may subtly affect the cupcake’s taste.

This substitute works well for dairy-free diets, producing cupcakes with good rise and moist crumb, though flavor nuances will vary based on the milk chosen.

Vegan Buttermilk Substitutes for Cupcakes

Full Vegan guide →
Non-Dairy Milk (Almond, Soy) + Lemon Juice or Vinegar
Ratio: 1 cup non-dairy milk + 1 tablespoon lemon juice or white vinegar (let sit 5-10 minutes)

Suitable dairy-free substitute that provides acidity but may impart slight nutty or bean flavor depending on milk used.

Gluten-Free Buttermilk Substitutes for Cupcakes

Full Gluten-Free guide →
Plain Yogurt (thinned with water or milk)
Ratio: 3/4 cup plain yogurt + 1/4 cup water or milk per 1 cup buttermilk

Thinning yogurt approximates buttermilk’s consistency and acidity, maintaining moisture and crumb softness.

Sour Cream (thinned with water or milk)
Ratio: 3/4 cup sour cream + 1/4 cup water or milk per 1 cup buttermilk

Thinning sour cream reduces density, helping maintain cupcake lightness while providing acidity.

Dairy-Free Buttermilk Substitutes for Cupcakes

Full Dairy-Free guide →
Non-Dairy Milk (Almond, Soy) + Lemon Juice or Vinegar
Ratio: 1 cup non-dairy milk + 1 tablespoon lemon juice or white vinegar (let sit 5-10 minutes)

Suitable dairy-free substitute that provides acidity but may impart slight nutty or bean flavor depending on milk used.

❌ What NOT to Use as a Buttermilk Substitute in Cupcakes

Plain Milk

Plain milk lacks the acidity needed to activate baking soda, which can result in cupcakes that are dense and less tender. Without the acid, the chemical leavening is incomplete, negatively impacting texture and rise.

Yogurt (without thinning)

Using thick yogurt without thinning can lead to overly dense and heavy cupcakes because it adds too much viscosity and alters the batter consistency, preventing proper aeration and rise.

Sour Cream (in equal volume)

Sour cream is much thicker and richer than buttermilk; substituting it in equal volume without adjustment can make cupcakes too dense and greasy, affecting crumb structure and mouthfeel.

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